TY - JOUR
T1 - 2-Deoxyribonolactone Lesions in X-Ray-Irradiated DNA: Quantitative Determination by Catalytic 5-Methylene-2-Furanone Release
AU - Roginskaya, Marina
AU - Razskazovskiy, Yuriy
AU - Bernhard, William A.
PY - 2005/9/26
Y1 - 2005/9/26
N2 - (Chemical Equation Presented) Torn genes: DNA tetramers containing the 2-deoxyribonolactone (dL) lesion have been isolated by HPLC from d(CGCG) and d(pCGCG) films irradiated with X-rays. Upon treatment with spermine as a catalyst, the dL-containing tetramers decompose to 5-methylene-2-furanone (5-MF; see scheme), a characteristic product of dL decomposition. Hence, 5-MP can be used to quantify dL lesions in DNA.
AB - (Chemical Equation Presented) Torn genes: DNA tetramers containing the 2-deoxyribonolactone (dL) lesion have been isolated by HPLC from d(CGCG) and d(pCGCG) films irradiated with X-rays. Upon treatment with spermine as a catalyst, the dL-containing tetramers decompose to 5-methylene-2-furanone (5-MF; see scheme), a characteristic product of dL decomposition. Hence, 5-MP can be used to quantify dL lesions in DNA.
KW - DNA
KW - DNA cleavage
KW - X-rays
KW - oligonucleotides
UR - https://dc.etsu.edu/etsu-works/18360
UR - https://doi.org/10.1002/anie.200501956
U2 - 10.1002/anie.200501956
DO - 10.1002/anie.200501956
M3 - Article
VL - 44
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
ER -