Development of a Method for the Recovery of Enteroviruses from Aerobically Digested Wastewater Sludges

Phillip R. Scheuerman; Samuel R. Farrah; Gabriel Bitton

doi:10.1016/0043-1354(86)90208-3

Development of a Method for the Recovery of Enteroviruses from Aerobically Digested Wastewater Sludges

Phillip R. Scheuerman
, Samuel R. Farrah
, Gabriel Bitton

University of Florida

Research output: Contribution to journal › Article › peer-review

Abstract

 A method was developed for virus recovery from aerobically digested sludge. This method, hereafter named the TCA-lysine procedure, consists of eluting virus from sludge with sodium trichloroacetate + lysine at pH 9.0. The eluates are further processed by two successive concentration steps.
 
 Comparison of this procedure was made to other methods. This procedure recovered 82% of added poliovirus from aerobic sludges. The range for the other procedures tested ranged from 1.1 to 66% recovery. Recovery of indigenous enteroviruses was found to be equal to or greater than other methods tested for both aerobically and anaerobically digested sludges.
 
 The TCA-lysine procedure is potentially more sensitive and cost effective than other methods tested due to the smaller concentration volume. In addition bacterial contamination of cell cultures used for viral assays was not as severe as with other procedures tested.

Original language	American English
Journal	Water Research
Volume	20
DOIs	https://doi.org/10.1016/0043-1354(86)90208-3
State	Published - 1986

Keywords

enteroviruses
wastewater sludges

Disciplines

Environmental Microbiology and Microbial Ecology

Access to Document

10.1016/0043-1354(86)90208-3License: Unspecified

Cite this

@article{e3a042d877574e358a01cf8585bfaf32,

title = "Development of a Method for the Recovery of Enteroviruses from Aerobically Digested Wastewater Sludges",

abstract = " A method was developed for virus recovery from aerobically digested sludge. This method, hereafter named the TCA-lysine procedure, consists of eluting virus from sludge with sodium trichloroacetate + lysine at pH 9.0. The eluates are further processed by two successive concentration steps. Comparison of this procedure was made to other methods. This procedure recovered 82\% of added poliovirus from aerobic sludges. The range for the other procedures tested ranged from 1.1 to 66\% recovery. Recovery of indigenous enteroviruses was found to be equal to or greater than other methods tested for both aerobically and anaerobically digested sludges. The TCA-lysine procedure is potentially more sensitive and cost effective than other methods tested due to the smaller concentration volume. In addition bacterial contamination of cell cultures used for viral assays was not as severe as with other procedures tested.",

keywords = "enteroviruses, wastewater sludges",

author = "Scheuerman, \{Phillip R.\} and Farrah, \{Samuel R.\} and Gabriel Bitton",

year = "1986",

doi = "10.1016/0043-1354(86)90208-3",

language = "American English",

volume = "20",

journal = "Water Research",

}

TY - JOUR

T1 - Development of a Method for the Recovery of Enteroviruses from Aerobically Digested Wastewater Sludges

AU - Scheuerman, Phillip R.

AU - Farrah, Samuel R.

AU - Bitton, Gabriel

PY - 1986

Y1 - 1986

N2 - A method was developed for virus recovery from aerobically digested sludge. This method, hereafter named the TCA-lysine procedure, consists of eluting virus from sludge with sodium trichloroacetate + lysine at pH 9.0. The eluates are further processed by two successive concentration steps. Comparison of this procedure was made to other methods. This procedure recovered 82% of added poliovirus from aerobic sludges. The range for the other procedures tested ranged from 1.1 to 66% recovery. Recovery of indigenous enteroviruses was found to be equal to or greater than other methods tested for both aerobically and anaerobically digested sludges. The TCA-lysine procedure is potentially more sensitive and cost effective than other methods tested due to the smaller concentration volume. In addition bacterial contamination of cell cultures used for viral assays was not as severe as with other procedures tested.

AB - A method was developed for virus recovery from aerobically digested sludge. This method, hereafter named the TCA-lysine procedure, consists of eluting virus from sludge with sodium trichloroacetate + lysine at pH 9.0. The eluates are further processed by two successive concentration steps. Comparison of this procedure was made to other methods. This procedure recovered 82% of added poliovirus from aerobic sludges. The range for the other procedures tested ranged from 1.1 to 66% recovery. Recovery of indigenous enteroviruses was found to be equal to or greater than other methods tested for both aerobically and anaerobically digested sludges. The TCA-lysine procedure is potentially more sensitive and cost effective than other methods tested due to the smaller concentration volume. In addition bacterial contamination of cell cultures used for viral assays was not as severe as with other procedures tested.

KW - enteroviruses

KW - wastewater sludges

UR - https://doi.org/10.1016/0043-1354(86)90208-3

U2 - 10.1016/0043-1354(86)90208-3

DO - 10.1016/0043-1354(86)90208-3

M3 - Article

VL - 20

JO - Water Research

JF - Water Research

ER -

Development of a Method for the Recovery of Enteroviruses from Aerobically Digested Wastewater Sludges

Abstract

Keywords

Disciplines

Access to Document

Other files and links

Cite this