HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration

Robert Wondergem; Tom W. Ecay; Frank Mahieu; Grzegorz Owsianik; Bernd Nilius

doi:10.1016/j.bbrc.2008.05.032

HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration

Robert Wondergem
, Tom W. Ecay
, Frank Mahieu
, Grzegorz Owsianik
, Bernd Nilius

Department of Biomedical Sciences

Quillen-Dishner College of Medicine
KU Leuven
Departement Cellulaire en Moleculaire Geneeskunde

Research output: Contribution to journal › Article › peer-review

Abstract

This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase [Ca2+]i needed for DBTRG cell migration.

Original language	American English
Journal	Biochemical and Biophysical Research Communications
Volume	372
DOIs	https://doi.org/10.1016/j.bbrc.2008.05.032
State	Published - Jul 18 2008

Keywords

DBTRG
HGF/SF
TRPM8
calcium
glioblastoma
hepatocyte growth factor/scatter factor
migration
transient receptor potential ion channel

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@article{0b51c2151669473594f9af861d472eb3,

title = "HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration",

abstract = " This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase [Ca2+]i needed for DBTRG cell migration.",

keywords = "DBTRG, HGF/SF, TRPM8, calcium, glioblastoma, hepatocyte growth factor/scatter factor, migration, transient receptor potential ion channel",

author = "Robert Wondergem and Ecay, \{Tom W.\} and Frank Mahieu and Grzegorz Owsianik and Bernd Nilius",

year = "2008",

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doi = "10.1016/j.bbrc.2008.05.032",

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T1 - HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration

AU - Wondergem, Robert

AU - Ecay, Tom W.

AU - Mahieu, Frank

AU - Owsianik, Grzegorz

AU - Nilius, Bernd

PY - 2008/7/18

Y1 - 2008/7/18

N2 - This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase [Ca2+]i needed for DBTRG cell migration.

AB - This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase [Ca2+]i needed for DBTRG cell migration.

KW - DBTRG

KW - HGF/SF

KW - TRPM8

KW - calcium

KW - glioblastoma

KW - hepatocyte growth factor/scatter factor

KW - migration

KW - transient receptor potential ion channel

UR - https://dc.etsu.edu/etsu-works/17410

UR - https://doi.org/10.1016/j.bbrc.2008.05.032

U2 - 10.1016/j.bbrc.2008.05.032

DO - 10.1016/j.bbrc.2008.05.032

M3 - Article

VL - 372

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

ER -

HGF/SF and Menthol Increase Human Glioblastoma Cell Calcium and Migration

Abstract

Keywords

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