Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase

Aruna Kilaru; Giorgis Isaac; Pamela Tamura; David Baxter; Scott R. Duncan; Barney J. Venables; Ruth Welti; Peter Koulen; Kent D. Chapman

doi:10.1007/s11745-010-3457-5

Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase

Aruna Kilaru
, Giorgis Isaac
, Pamela Tamura
, David Baxter
, Scott R. Duncan
, Barney J. Venables
, Ruth Welti
, Peter Koulen
, Kent D. Chapman

University of North Texas
Kansas State University
University of Missouri–Kansas City

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-5"> N &hyphen;Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon ( N &hyphen;acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase ( FAAH −/− ) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of  FAAH −/−  mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.</div>

Original language	American English
Journal	Lipids
Volume	45
DOIs	https://doi.org/10.1007/s11745-010-3457-5
State	Published - Aug 17 2010
Externally published	Yes

Disciplines

Biochemistry

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Cite this

@article{1c26548e786f44a8913e0c023d8b1e3d,

title = "Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase",

abstract = " N \&hyphen;Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon ( N \&hyphen;acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase ( FAAH \−/\− ) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of\  FAAH \−/\− \ mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.",

author = "Aruna Kilaru and Giorgis Isaac and Pamela Tamura and David Baxter and Duncan, \{Scott R.\} and Venables, \{Barney J.\} and Ruth Welti and Peter Koulen and Chapman, \{Kent D.\}",

year = "2010",

month = aug,

day = "17",

doi = "10.1007/s11745-010-3457-5",

language = "American English",

volume = "45",

journal = "Lipids",

}

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T1 - Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase

AU - Kilaru, Aruna

AU - Isaac, Giorgis

AU - Tamura, Pamela

AU - Baxter, David

AU - Duncan, Scott R.

AU - Venables, Barney J.

AU - Welti, Ruth

AU - Koulen, Peter

AU - Chapman, Kent D.

PY - 2010/8/17

Y1 - 2010/8/17

N2 - N &hyphen;Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon ( N &hyphen;acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase ( FAAH −/− ) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of  FAAH −/−  mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.

AB - N &hyphen;Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon ( N &hyphen;acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase ( FAAH −/− ) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of  FAAH −/−  mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.

U2 - 10.1007/s11745-010-3457-5

DO - 10.1007/s11745-010-3457-5

M3 - Article

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JO - Lipids

JF - Lipids

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