Mapping of the Two Resistance Genes rip and pal1 by in situ Hybridization

In this paper, the low copy genes rip and pal1 were physically mapped by in situ bybridization. With biotinylated cDNA probes of the two genes and DAB detection, the signals of the rip gene were detected in the long arms of the eighth and third chromosomes. The percent distances from centromere to detection site were respectively 23.66±1.32, 72.47±3.16. And the pal1 gene was located in the long arms of both the fifth and fourth chromosomes, the short arm of the second chromosome. The percent distances were respectively 20.85±1.08, 61.63±2.21, 28.23±0.82.